{"product_id":"bovine-calpain-2-catalytic-subunit-capn2-5ug","title":"Bovine Calpain-2 Catalytic Subunit, CAPN2, 5ug","description":"\u003ctable width=\"100%\" cellspacing=\"0\" cellpadding=\"0\" border=\"0\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan style=\"font-weight: bold; font-family: Calibri; font-size: 10pt; color: rgb(0, 128, 0);\"\u003eCAT No.BT-PRO-330\u003c\/span\u003e\u003c\/td\u003e                                    \u003c\/tr\u003e                                   \u003ctr\u003e                                                \u003ctd\u003e    \u003cspan style=\"font-family: Arial; font-weight: 700; font-size: 12pt; color: rgb(128, 0, 0);\"\u003eBovine Calpain-2 Catalytic Subunit, CAPN2, 5ug\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003chr\u003e\n\u003cp\u003eApplications\u003cbr\u003eThis protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.\u003cbr\u003eFor adsorption we suggest the following procedureA- Dilute 1?l of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry (final dilution 1:1'000).\u003cbr\u003eB- Add 1ug of protein to 1ml of the diluted antibody solution and mix well.\u003cbr\u003eC- Incubate for at least 6 hours in the cold.\u003cbr\u003eD- Apply to tissue-sections and incubate for 3 days.\u003cbr\u003eE - Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).\u003cbr\u003eAs a result, the immunostaining should be strongly reduced or even completely prevented.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eDescription\u003cbr\u003eCAPN2 consists of an 80 kDa large subunit and a 30 kDa small subunit. CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, and Phenyl-Sepharose CL-4B columns.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eFormulation\u003cbr\u003e50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT and 50% glycerol, pH 7.4.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eSterile Filtered colorless solution.\u003cp\u003e\u003c\/p\u003e\u003cp\u003ePurity\u003cbr\u003eGreater than 90% as determined by SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eSource\u003cbr\u003eBovine Myocardium.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eStability\u003cbr\u003eCAPN2 although stable at 10¡ãC for 1 week, should be stored desiccated below -18¡ãC. \u003cbr\u003eFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).\u003cbr\u003ePlease prevent freeze-thaw cycles.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eSynonyms\u003cbr\u003eCalpain-2 catalytic subunit, EC 3.4.22.53, Calpain-2 large subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, M-calpain, Millimolar-calpain, CAPN2.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eUsage\u003cbr\u003eTSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e","brand":"My Store","offers":[{"title":"Default Title","offer_id":42034553552993,"sku":"BT-PRO-330","price":1756.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0567\/9595\/5297\/files\/recom_protein_p_68717e67-3da6-487b-b64c-70020b544b18.jpg?v=1741097843","url":"https:\/\/biotangusa.com\/products\/bovine-calpain-2-catalytic-subunit-capn2-5ug","provider":"Biotang USA","version":"1.0","type":"link"}