{"product_id":"fibronectin-human-fibronectin-human-1mg","title":"Fibronectin Human, Fibronectin Human, 1mg","description":"\u003ctable width=\"100%\" cellspacing=\"0\" cellpadding=\"0\" border=\"0\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan style=\"font-weight: bold; font-family: Calibri; font-size: 10pt; color: rgb(0, 128, 0);\"\u003eCAT No.BT-PRO-448\u003c\/span\u003e\u003c\/td\u003e                                    \u003c\/tr\u003e                                   \u003ctr\u003e                                                \u003ctd\u003e    \u003cspan style=\"font-family: Arial; font-weight: 700; font-size: 12pt; color: rgb(128, 0, 0);\"\u003eFibronectin Human, Fibronectin Human, 1mg\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003chr\u003e\n\u003cp\u003eCell Culture Application\u003cbr\u003eFibronectin is useful for the induction of cell attachment to a variety of surfaces including plastic and glass tissue culture labware, petri dishes, coverslips, microcarrier beads, etc. Fibronectin is useful for growth and maintenance of cells in low serum conditions. In general, the reconstituted fibronectin should be diluted with sterile physiological saline or serum-free medium to a concentration of 10-50ug\/ml. The plates are treated with the solution, at room temperature 1-2 hours, the supernatant removed and the plates rinsed with fresh media or buffer. Growth media is then added and the coated plates seeded with a suspension of freshly harvested cells. Care should be taken to inactivate serum proteases used in the preparation of the cells, since fibronectin is very sensitive to proteolysis. Optimum concentration of fibronectin depends on the types of cell and the particular surface used. Generally, use 1-5ug\/cm? of growth surface.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eFormulation\u003cbr\u003eThe Fibronectin was lyophilized from a concentrated 1mg\/ml solution containing 0.05M Tris Cl pH-7.5 and 0.1M NaCl.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003ePhysical Appearance\u003cbr\u003eSterile Filtered White lyophilized (freeze-dried) powder.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eGreater than 95.0% as determined by SDS-PAGE.\u003cp\u003e\u003c\/p\u003e\u003cp\u003eSolubility\u003cbr\u003ePurified fibronectin has a tendency to form insoluble aggregates upon reconstitution. We suggest to reconstittue the 1mg Fibronectin with a chaotropic agent such as urea at room temperature at a concentration of 0.2mg\/ml using sterile water. \u003cbr\u003eLet stand 1-2 hours.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eSource\u003cbr\u003eHuman Plasma.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eStability\u003cbr\u003eLyophilized Fibronectin although stable at room temperature for 1 week, should be stored desiccated below -18¡ãC. \u003cbr\u003eUpon reconstitution Fibronectin should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.\u003cbr\u003ePlease prevent freeze-thaw cycles.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eUsage\u003cbr\u003eTSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e","brand":"My Store","offers":[{"title":"Default Title","offer_id":42035435372641,"sku":"BT-PRO-448","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0567\/9595\/5297\/files\/recom_protein_p_57006e7d-d953-4fdf-95f4-c3724e006c89.jpg?v=1741122621","url":"https:\/\/biotangusa.com\/products\/fibronectin-human-fibronectin-human-1mg","provider":"Biotang USA","version":"1.0","type":"link"}