{"product_id":"human-calpain-1-catalytic-subunit-capn1-5ug","title":"Human Calpain-1 Catalytic Subunit, CAPN1, 5ug","description":"\u003ctable width=\"100%\" cellspacing=\"0\" cellpadding=\"0\" border=\"0\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan style=\"font-weight: bold; font-family: Calibri; font-size: 10pt; color: rgb(0, 128, 0);\"\u003eCAT No.BT-PRO-363\u003c\/span\u003e\u003c\/td\u003e                                    \u003c\/tr\u003e                                   \u003ctr\u003e                                                \u003ctd\u003e    \u003cspan style=\"font-family: Arial; font-weight: 700; font-size: 12pt; color: rgb(128, 0, 0);\"\u003eHuman Calpain-1 Catalytic Subunit, CAPN1, 5ug\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003chr\u003e\n\u003cp\u003eApplications\u003cbr\u003eThis protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.\u003cbr\u003eFor adsorption we suggest the following procedure:\r\nA- Dilute 1?l of the antiserum against ?-calpain in 1ml of the usual buffer for immunohistochemistry (final dilution 1:1000).\r\nB- Add 1ug of protein to 1ml of the diluted antibody solution and mix well.\r\nC- Incubate for at least 6 hours in the cold.\r\nD- Apply to tissue-sections and incubate for 3 days.\r\nE - Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).\r\nAs a result, the immunostaining should be strongly reduced or even completely prevented.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eDescription\u003cbr\u003eCAPN1 consists of an 80-kDa large subunit and a 30 kDa small subunit. \u003cbr\u003eCAPN1 was purified by sequential chromatography through DEAE-Sepharose, A1.5m Bio-Gel, and Phenyl-Sepharose CL-4B columns.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eFormulation\u003cbr\u003e50mM imidazole-HCl, 100mM NaCl, 5mM EGTA, 1mM DTT and 10% sucrose.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eSterile Filtered colorless solution.\u003cp\u003e\u003c\/p\u003e\u003cp\u003ePurity\u003cbr\u003eGreater than 90% as determined by SDS-PAGE.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eSource\u003cbr\u003eHuman Erythrocytes.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eStability\u003cbr\u003eCAPN1 although stable at 10¡ãC for 1 week, should be stored desiccated below -18¡ãC. \u003cbr\u003eFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).\u003cbr\u003ePlease prevent freeze-thaw cycles.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eSynonyms\u003cbr\u003eCalpain-1 catalytic subunit, EC 3.4.22.52, Calpain-1 large subunit, Calcium-activated neutral proteinase 1, Calpain mu-type, muCANP, Micromolar-calpain, Cell proliferation-inducing gene 30 protein, CANP 1, CAPN1, CANPL1, PIG30, CANP, muCL, CANP1.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eUsage\u003cbr\u003eTSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e","brand":"My Store","offers":[{"title":"Default Title","offer_id":42034554699873,"sku":"BT-PRO-363","price":1756.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0567\/9595\/5297\/files\/recom_protein_p_c0621577-6afd-48dc-ad40-19fa51ef9f8e.jpg?v=1741097872","url":"https:\/\/biotangusa.com\/products\/human-calpain-1-catalytic-subunit-capn1-5ug","provider":"Biotang USA","version":"1.0","type":"link"}