{"product_id":"modified-affinity-purified-avidin-avidin-biotin-10mg","title":"Modified Affinity Purified Avidin, Avidin Biotin, 10mg","description":"\u003ctable width=\"100%\" cellspacing=\"0\" cellpadding=\"0\" border=\"0\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan style=\"font-weight: bold; font-family: Calibri; font-size: 10pt; color: rgb(0, 128, 0);\"\u003eCAT No.BT-PRO-446\u003c\/span\u003e\u003c\/td\u003e                                    \u003c\/tr\u003e                                   \u003ctr\u003e                                                \u003ctd\u003e    \u003cspan style=\"font-family: Arial; font-weight: 700; font-size: 12pt; color: rgb(128, 0, 0);\"\u003eModified Affinity Purified Avidin, Avidin Biotin, 10mg\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003chr\u003e\n\u003cp\u003eActivity\u003cbr\u003eBinding activity of Avidin-Biotin is 10-15units\/mg protein. \u003cbr\u003eOne unit of Avidin activity is defined as the amount of protein which will bind 1ug d-biotin.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eAdvantages\u003cbr\u003e1. Avidin-Biotin does not exhibit unwanted non-specific binding that have been previously reported for the native egg white avidin.\r\n2. The non-specific properties of Avidin-Biotin have been tested in ELISA, Western Blot \u0026amp; Immunohistochemistry techniques and have been found to be superior to unmodified avidin. \r\n3. Avidin-Biotin rarely affected by pH values between the range of pH-2 to pH-12 or by guanidine-HCl concentrations up to 8M at neutral pH.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eApplications\u003cbr\u003eAvidin-Biotin may be used in combination with biotin in various techniques such as ELISA, Western blotting, immunohistochemistry etc¡­\r\n\r\n\r\n\r\nPhysical AppearanceLyophilized freeze dry Powder.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003eAvidin is a basic charged glycoprotein present in the egg white and in some extent in tissues of various animals. Avidin is a homotetrameric protein that contains 4 identical subunits which bind to Biotin with high extent of affinity and specificity consisting of 128 amino acids and has a molecular mass of about 67K Daltons. \u003cbr\u003eAvidin-Biotin is a modified form of affinity purified avidin that combines the high affinity specific activity of the native egg white avidin to biotin molecule together with a very low background of the streptavidin produced by the bacteria Streptomyces avidinii.\u003cp\u003e\u003c\/p\u003e\u003cp\u003eFormulation\u003cbr\u003eSalt-free lyophilized powder with no preservative added.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eSolubility\u003cbr\u003eWe highly recommended to prepare a 1 mg\/ml solution; Reconstitute using cold deionized pyrogen free water and mix gently until powder is completely dissolved.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eStability\u003cbr\u003eStore protein solution at 2-8¡ãC.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eSynonyms\u003cbr\u003eAvidin, AVD, AVID.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e\u003cp\u003eUsage\u003cbr\u003eTSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.\u003c\/p\u003e\u003cp\u003e\u003c\/p\u003e","brand":"My Store","offers":[{"title":"Default Title","offer_id":42035434881121,"sku":"BT-PRO-446","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0567\/9595\/5297\/files\/recom_protein_p_0628a3e4-8dec-4b0f-8079-9771dba09f07.jpg?v=1741122593","url":"https:\/\/biotangusa.com\/products\/modified-affinity-purified-avidin-avidin-biotin-10mg","provider":"Biotang USA","version":"1.0","type":"link"}