| CAT No.BT-ENZ-084 |
| 2-Oxo-Acid Dehydrogenase Complex E2 Human Recombinant, BCOADC-E2 Human, 10ug |
Applications
Western blot with anti-M2-Antigen autoantibody-positive patient sera or monoclonal
anti-hexa-His-tag antibody.
Coating concentration
0.4-0.8 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Description
BCOADC-E2 is a Full-length cDNA coding for the mature form of the human BCOADC-E2 protein having a molecular mass of 47,321 Dalton(pH 6.5). BCOADC-E2 protein is fused to a hexa-histidine purification tag.
DLST (0.91mg/ml) is supplied in 20mM HEPES buffer pH-8.0, 200mM NaCl and 20% glycerol.
Immunological function
Binds IgG-type human auto-antibodies.
Immunological tests
Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test with positive/negative sera panels.
Purity
Greater than 75% as determined by SDS-PAGE.
Source
Sf9 insect cells.
Storage Conditions
Store at 4¡ãC if entire vial will be used within 2-4 weeks. Store, frozen at -20¡ãC for longer periods of time.
Avoid multiple freeze-thaw cycles.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
