| CAT No.BT-PKA-312 |
| Active Focal Adhesion Kinase Human Recombinant, FAK1 Human, 5ug |
Amino Acid Sequence
MAPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRHNQTSL YKKAGTMAAA YLDPNLNHTP NSSTKTHLGT GMERSPGAME RVLKVFHYFE SNSEPTTWAS IIRHGDATDV
RGIIQKIVDS HKVKHVACYG FRLSHLRSEE VHWLHVDMGV SSVREKYELA HPPEEWKYEL RIRYLPKGFL NQFTEDKPTL NFFYQQVKSD YMLEIADQVD QEIALKLGCL EIRRSYWEMR GNALEKKSNY EVLEKDVGLK RFFPKSLLDS VKAKTLRKLI QQTFRQFANL NREESILKFF EILSPVYRFD KECFKCALGS SWIISVELAI GPEEGISYLT DKGCNPTHLA DFTQVQTIQY SNSEDKDRKG MLQLKIAGAP EPLTVTAPSL TIAENMADLI DGYCRLVNGT SQSFIIRPQK
EGERALPSIP KLANSEKQGM RTHAVSVSET DDYAEIIDEE DTYTMPSTRD YEIQRERIEL GRCIGEGQFG DVHQGIYMSP ENPALAVAIK TCKNCTSDSV REKFLQEALT MRQFDHPHIV KLIGVITENP VWIIMELCTL GELRSFLQVR KYSLDLASLI LYAYQLSTAL AYLESKRFVH RDIAARNVLV SSNDCVKLGD FGLSRYMEDS TYYKASKGKL PIKWMAPESI NFRRFTSASD VWMFGVCMWE ILMHGVKPFQ GVKNNDVIGR IENGERLPMP PNCPPTLYSL MTKCWAYDPS
RRPRFTELKA QLSTILEEEK AQQEERMRME SRRQATVSWD SGGSDEAPPK PSRPGYPSPR SSEGFYPSPQ HMVQTNHYQV SGYPGSHGIT AMAGSIYPGQ ASLLDQTDSW NHRPQEIAMW QPNVEDSTVL DLRGIGQVLP THLMEERLIR QQQEMEEDQR WLEKEERFLK PDVRLSRGSI DREDGSLQGP IGNQHIYQPVGKPDPAAPPK KPPRPGAPGH LGSLASLSSP ADSYNEGVKL QPQEISPPPT ANLDRSNDKV YENVTGLVKA VIEMSSKIQP APPEEYVPMV KEVGLALRTL
LATVDETIPL LPASTHREIE MAQKLLNSDL GELINKMKLA QQYVMTSLQQ EYKKQMLTAA HALAVDAKNL LDVIDQARLK MLGQTRPH.
Biological Activity
72 nmole of phosphate transferred to poly [Glu,Tyr] 4:1 substrate/minute/mg of total protein at 30¡ãC. Activity determined at a final protein concentration of 4¦Ìg/ml.
Description
Active Focal Adhesion Kinase full length Human Recombinant is a 146.7 kDa enzyme produced in Sf9 cells with an amino-terminal GST tag and purified via sequential chromatography. PTK2 is activated in-vitro by his tagged SRC.
20mM Tris pH-7.5, 10% Glycerol, 0.02% Triton X-100, 0.1mg/ml BSA, 0.5mM Na3VO4, 2mM DTT.
Formulation
FADK is supplied in 50mM Tris pH-7.5, 150mM NaCl, 0.5mM EDTA, 0.02% Triton X-100, 2mM DTT, & 50% Glycerol.
Purity
Greater than 70% as determined by SDS-PAGE.
Source
Baculovirus, SF9 insect cells.
Storage Conditions
Store at 4¡ãC if entire vial will be used within 1-2 weeks.
Store, frozen at -20¡ãC to -80¡ãC for longer periods of time.
Avoid multiple freeze-thaw cycles.
Synonyms
Focal adhesion kinase 1, EC 2.7.10.2, FADK 1, pp125FAK, Protein-tyrosine kinase 2, FAK, FADK, FAK1.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
