| CAT No.BT-ENZ-353 |
| Adenosine 5" Triphosphate Sulfurylase Yeast Recombinant, ATP sulfurylase Yeast, 50U |
Amino Acid Sequence
MPAPHGGILQ DLIARDALKK NELLSEAQSS DILVWNLTPR QLCDIELILN GGFSPLTGFL NENDYSSVVT DSRLADGTLW TIPITLDVDE AFANQIKPDT RIALFQDDEI PIAILTVQDV YKPNKTIEAE KVFRGDPEHP AISYLFNVAG DYYVGGSLEA IQLPQHYDYP GLRKTPAQLR LEFQSRQWDR VVAFQTRNPM HRAHRELTVR AAREANAKVL IHPVVGLTKP GDIDHHTRVR VYQEIIKRYP NGIAFLSLLP LAMRMSGDRE AVWHAIIRKN YGASHFIVGR DHAGPGKNSK GVDFYGPYDA QELVESYKHE DIEVVPFRM VTYLPDEDRY APIDQIDTTK TRTLNISGTE LRRRLRVGGE IPEWFSYPEV VKILRESNPP RPKQGFSIVL GNSLTVSREQ LSIALLSTFL QFGGGRYYKI FEHNNKTELL SLIQDFIGSG SGLIIPNQWE DDKDSVVGKQ NVYLLDTSSS ADIQLESADE PISHIVQKVV LFLEDNGFFV F.
Biological Activty
12 Units/mg.
Description
Adenosine 5" Triphosphate Sulfurylase Yeast Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain containg 511 amino acids and having a Mw of 57.7 kDa. Adenosine 5" Triphosphate Sulfurylase Yeast Recombinant catalyzes the activation of sulfate by transferring sulfate to the adenine monophosphate moiety of ATP to form adenosine 5?-phosphosulfate (APS) and pyrophosphate (PPi). The reaction is reversible: ATP is formed from APS and PPi.
Adenosine 5 Triphosphate Sulfurylase is purified by proprietary chromatographic techniques.
The ATP sulphurylase protein was lyophilized after dialysis against lyophilized from 10mM NaP buffer, 100mM NaCl, 10mM Lactose, 1% PEG pH 7.5 and 0.75mM DTT.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Solubility
Spin vial before opening. Reconstitute ATP sulphurylase with 5mM NaP pH-7.5 & 0.75mM DTT at a concentration ranging from 0.1mg ¨C 1mg per ml. Can be diluted further into other aqueous buffers. pH range between 7.0 ¨C 8.5 is best.
Source
Escherichia Coli.
Stability
Lyophilized Sulfate adenylate transferase although stable at 4¡ãC for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution ATPS should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
Sulfate adenylyltransferase, EC 2.7.7.4, Sulfate adenylate transferase, SAT, ATP-sulfurylase, Methionine-requiring protein 3, ATPS.
Unit Definition
One unit produces 1.0 ?mole of ATP from APS and inorganic phosphate per min. at pH 8.0 and 30¡ãC.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
