Agarose LE (Low
Electroendosmosis), BiotechGrade, 500g
AGAROSE LE
High Performance, General Purpose Agarose
- Multi-purpose, high purity Enhanced resolution and
clarity
- Reduced background
- Low EEO/increased electrophoretic mobility
- RNase, DNase, Protease-free
- High hysteresis, improved thermal stability
Benchmark Agarose LE is a highly purified agarose, suitable
for a variety of molecular biology applications. It Is refined using an advanced
process that excludes the use of organic solvents, yielding a cleaner
end-product with a significantly reduced environmental impact. Agarose LE can be
used for analyses of proteins and nucleic acids of various sizes (150 bp to 6
kbp).
It's low EEO
(<
0.13-mr)
promotes increased electrophoretic mobility, yielding improved resolution and
shorter run times. This also allows macromolecules and larger particles (subcellular
fragments, viruses, etc) to migrate more freely through the gel matrix. The
consistently low EEO also provides a reduction in band distortion (caused by
counterflow) that can result from the presence of excessive sulfate-rich
negative ions.
Agarose LE is widely used for
nucleic
acid electrophoresis (analytical and
preparative), protein electrophoresis (including radial immunodiffusion) and
various blotting protocols. It
is easily soluble, free
of nucleases, and easy to use. It is highly transparent (forms a clear,
colorless solution at 1g: 100ml Hp), and exhibits exceptionally low absorption
of chemical staining agents. Pore size can be adjusted by simple modifications
to the concentration ratio.
Formulated for high gel strength and integrity, Agarose LE
exhibits exceptional thermal stability and mechanical resistance, ensuring safe,
easy handling, regardless of whether a denaturing agent has been added.
