| CAT No.BT-PKA-305 |
| AKT1 Human Recombinant, AKT1 Human, 10ug |
Description
AKT1 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain containing 481 amino acids and having a molecular mass of 60 KD. This protein is the full-length form of the protein with a N-terminal His-tag.
AKT1 is purified by proprietary chromatographic techniques.
Formulation
AKT1 (0.1mg/ml) is supplied in 50mM Tris pH 7.5, 0.15M NaCl, 0.27M sucrose,10mM beta-mercaptoethanol, 1mM EGTA. Carrier-free.
Physical Appearance
Sterile Filtered clear solution.
:Kinase activity may vary depending on the substrate and reaction conditions.
Purity
: Greater than 95% as determined by SDS-PAGE.
Mass spectrometry of tryptic digest products for identity determination.
Source
Baculovirus, SF9 insect cells.
Storage Conditions
Store at 4¡ãC if entire vial will be used within 1-2 weeks. Store, frozen at -20¡ãC- -80¡ãC for longer periods of time. Avoid multiple freeze-thaw cycles.
Synonyms
RAC-alpha serine/threonine-protein kinase, EC 2.7.11.1, RAC-PK-alpha, Protein kinase B, C-AKT, AKT, PKB, RAC, PRKBA, MGC99656, RAC-ALPHA.
Unit Definition
250 units/mg. One unit of Akt1 activity is equal to 1 nanomole of phosphate transferred to crosstide substrate per minute at 30¡ãC with a final ATP concentration of 100?M. The optimal concentration should be determined for each specific application.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
