| CAT No.BT-ENZ-275 |
| Aminopeptidase Aeromonas Recombinant, Aminopeptidase, 250U |
Unit Defenition
One unit is defined as the amount of enzyme that hydrolyses 1?mole of L-leucine p-nitroanilide at 25¡ãC per 1 minute.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Biological Activity
Recombinant Aeromonas Aminopeptidase was found to have an activity of 120 Units/mg protein.
The 29 kDa Aeromonas Aminopeptidase is produced by genetic engineering and can be used for physical & structural investigations, sequence and amino-terminal determinations. This exopeptidase recognizes a specific stop sign at ¨CX- Pro and requires a free a-amino group in the L-configuration. It is therefore suitable for the removal of the redundant N-terminal methionine often added to engineered recombinant proteins.
Formulation
Buffered solution containing 10mM Tris-HCL, 100mM NaCl and 5?M ZnSO4, pH 8.0.
Physical Appearance
Sterile filtered liquid formulation.
Purity
Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Source
Aeromonas Proteolytica.
Stability
Two years when stored at ¨C20¡ãC, 2 weeks at 4¡ãC.
Synonyms
Bacterial leucyl aminopeptidase, EC 3.4.11.10.
