| CAT No.BT-PRO-284 |
| Angiostatin Kringles 1-3 Human Recombinant, Ang K1-3 Human, 100ug |
Amino Acid Sequence
VYLSECKTGN GKNYRGTMSK TKNGITCQKW SSTSPHRPRF SPATHPSEGL EENYCRNPDN DPQGPWCYTT DPEKRYDYCD ILECEEECMH CSGENYDGKI SKTMSGLECQ AWDSQSPHAH GYIPSKFPNK NLKKNYCRNP DRELRPWCFT TDPNKRWELC DIPRCTTPPP SSGPTYQCLKGTGENYRGNV AVTVSGHTCQ HWSAQTPHTH NRTPENFPCK NLDENYCRNP DGKRAPWCHT TNSQVRWEYC KIPSCDSSP.
Biological Activity
The activity is assayed on anti-proliferation and anti-migration of endothelial cells in vitro and anti-angiogenesis in vivo. The specific activity of anti-migration of endothelial cells in vitro is 55,000 Units/mg.
Description
Angiostatin Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 259 amino acids and having a molecular mass of approximately 30.0 kDa.
The Ang K1-3 is purified by proprietary chromatographic techniques.
Lyophilized from a 0.2¦Ìm filtered concentrated (1.0mg/ml) solution in 20mM NaAc, pH5.5, 4% mannitol.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Protein content
Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 2.08 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of Ang K1-3 as a Reference Standard.
Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility
We recommend to briefly centrifuge the vial prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20¡ãC. Further dilutions should be made in appropriate buffered solutions.
Source
Escherichia coli.
Stability
The lyophilized Angiostatin K1-3 is stable for several weeks at 2-8¡ãC, but should be kept at -20¡ãC for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8¡ãC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20¡ãC to -70¡ãC.
Avoid repeated freeze/thaw cycles.
Synonyms
Angiostatin, Angiostatin Kringles 1-3, Ang K1-3.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Human Virus Test
Starting material tested and found negative for HIV I & II antibodies, Hepatitis B surface antigen, and Hepatitis C antibodies. Finished product tested and found negative for HIV antigen P24.
