| CAT No.BT-PRO-411 |
| B-Cell Leukemia/Lymphoma 2 Human Recombinant (-BH4), Bcl 2 Human (minus BH4 domain), 10ug |
Applications
Input marker or positive control (Western Blotting).
Function study.
Description
Bcl-2 Des BH4 domain (10-30 residues) Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 197 amino acids 1-9 and 31-218.
The Bcl-2 is expressed as His-Tag fusion protein and purified by proprietary chromatographic techniques.
Formulation
The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl.
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility
Suspend Bcl-2 in 100?l of 0.5M Acetic acid, over night at 4¡ãC.
Dilute 10 fold into selected buffer system.
BCL-2 has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended.
Source
Escherichia Coli.
Stability
Lyophilized Bcl-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution Bcl-2 should be stored at 4¡ãC between 2-7 days and for future use below
-18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
Apoptosis regulator Bcl-2, BCL2, B-cell CLL/lymphoma 2, Bcl-2.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
