Brain-Derived Neurotrophic Factor Human Recombinant, BDNF Human, 10ug

Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Ser-Asp-Pro.

Biological Activity
The ED50, calculated by the dose-dependant induction of ACHE (acetylcholine esterase) in rat basal forebrain primary septal culture is 50 ng/ml corresponding to a Specific Activity of 20,000IU/mg.

Description
Brain-Derived Neurotrophic Factor Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 119 amino acids and having a total molecular mass of 26,984 Dalton.
BDNF Human Recombinant is purified by proprietary chromatographic techniques.

Formulation
The protein was lyophilized after dialysis against 20mM sodium citrate buffer pH=5.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Protein content
BDNF quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 1.6 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a standard solution of Brain-derived Neurotrophic Factor as a Reference Standard.

Purity
Greater than 96.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Brain-derived Neurotrophic Factor in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized BDNF although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution Brain-derived Neurotrophic Factor should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
BDNF, MGC34632.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.