| CAT No.BT-PRO-410 |
| Calsequestrin-2 Dog, CASQ2 Dog, 5ug |
Applications
Immunoblots
Absorption
Immunohistochemistry.
Description
Calsequestrin is the major calcium storage protein of the sarcoplasmic reticulum. Intraluminar Ca2+ binds to calsequestrin during diastole to prevent Ca2+ precipitation and to lower its free ionic concentration to facilitate efficient storage. During systole, Calsequestrin coordinately releases ~40?50 Ca2+ ions per molecule for each contraction-relaxation cycle by an uncertain mechanism. Calsequestrin has been shown to be of major importance in the regulation of cardiac excitation-contraction coupling.
Formulation
The protein was lyophilized from a concentrated solution (1mg/ml) containing 10mM Tris-HCl and 1mM EGTA.
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 90% as determined by SDS-PAGE.
Solubility
It is recommended to reconstitute the lyophilized CASQ2 in sterile 18M?-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Source
Dog Heart.
Stability
Lyophilized CASQ2 although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution CASQ2 should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
Calsequestrin-2, Calsequestrin cardiac muscle isoform, CASQ2, CSQ.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
