CAT No.BT-PRO-389 |
Centromere Protein A Human Recombinant, CENPA Human, 20ug |
Description
CENPA Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 17,015 Dalton.
CENPA is expressed with a -6x His tag and purified by proprietary chromatographic techniques.
Applications
Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.
Coating concentration
0.15-0.4 ug/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.
CENPA is supplied in 20mM HEPES buffer pH-8, 100mM sodium chloride, and 6M Urea.
Immunological functions
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
Physical Appearance
Sterile Filtered liquid formulation.
Purity
Greater than 90% as determined by SDS-PAGE.
Source
Sf9 insect cells.
Storage Conditions
Store at 4¡ãC if entire vial will be used within 2-4 weeks.
Store, frozen at -20¡ãC for longer periods of time.
Avoid multiple freeze-thaw cycles.
Synonyms
Histone H3-like centromeric protein A, Centromere protein A, CENP-A, Centromere autoantigen A, CENPA.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.