| CAT No.BT-ENZ-257 |
| Chaperone SURA E.Coli Recombinant, SURA E.Coli, 25ug |
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAPQVVDKVA AVVNNGVVLE SDVDGLMQSV KLNAAQARQQ LPDDATLRHQ IMERLIMDQI ILQMGQKMGV
KISDEQLDQA IANIAKQNNM TLDQMRSRLA YDGLNYNTYR NQIRKEMIIS EVRNNEVRRR ITILPQEVES LAQQVGNQND ASTELNLSHI
LIPLPENPTS DQVNEAESQA RAIVDQARNG ADFGKLAIAH SADQQALNGG QMGWGRIQEL PGIFAQALST AKKGDIVGPI RSGVGFHILK
VNDLRGESKN ISVTEVHARH ILLKPSPIMT DEQARVKLEQ IAADIKSGKT TFAAAAKEFS QDPGSANQGG DLGWATPDIF DPAFRDALTR
LNKGQMSAPV HSSFGWHLIE LLDTRNVDKT DAAQKDRAYR MLMNRKFSEE AASWMQEQRA SAYVKILSN.
Biological Activity
Specific activity is > 40 nmoles/min/ug, and is defined as the amount of enzyme that cleave 1umole of suc-AAFP-pNA per minute at 1C in Tris-Hcl pH8.0 using chymotrypsin.
Description
SURA E.Coli Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 429 amino acids (21-428 a.a.) and having a molecular wieght of 47.3 kDa. The SURA is fused to 21 a.a. His-Tag at N-terminus and purified by proprietary chromatographic techniques.
The SURA 1mg/ml protein solution contains 20mM Tris-HCl, pH-8, and 10% glycerol.
Physical Appearence
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Source
Escherichia Coli.
Stability
Store at 4¡ãC if entire vial will be used within 2-4 weeks. Store, frozen at -20¡ãC for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Synonyms
Rotamase surA, Survival protein A.
Usage
TSZGENE''s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
