| CAT No.BT-PRO-387 |
| Collagen Type IV Alpha 3 Human Recombinant, COL4A3 Human, 10ug |
Physical Appearance
Sterile Filtered clear solution.
Applications
Standard ELISA test
Immuno-Dot test with positive/negative sera panels.
SDS-PAGE
Western-Blot.
Caution
It has been reported that the immunodominant epitope of COL4A3 is a cryptic epitope that is not easily accessible to the corresponding autoantibodies. It is necessary to treat the protein under nonreducing conditions with a denaturant such as urea to unmask the epitopes (see Hellmark et al. in Autoantibodies, Peter, J.B. and Shoenfeld, Y., eds., Elsevier B.V., 1996, pp 291-298).
0.12-0.5 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Description
Human a3 chain of collagen IV; identical with the antigen called "glomerular basal membrane antigen" (GBM). Recombinant antigen for solid (ELISA) and fluid phase diagnostic assays. Binds IgG-type human auto-antibodies. Calculated Molecular weight: 43,591 Dalton. Calculated isoelectric point: pH 8.9. cDNA coding for a minicollagen version of the human collagen IV a3 chain fused to a hexa-histidine purification tag. The term minicollagen designates the removal of most of the epitope-less triplehelical collagenous region (situated between the N-terminal 7S domain and the C-terminal noncollagenous NC1 domain), which is a requirement for recombinant production of this antigen.
Formulation
The protein solution (0.6 mg/ml) contains 20mM Hepes, pH 8.0, and 4M Urea
Source
Sf9 insect cells.
Stability
Recommendations for storage buffer: Ionic strength between 50 and 100 mM, neutral to slightly alkaline pH and 4 M urea as dissociating agent.
Storage temperature is -70¡ã to -80¡ã C.
Please prevent freeze-thaw cycles.
Synonyms
Collagen alpha-3(IV) chain, Goodpasture antigen, COL4A3, Glomerular Basal Membrane, GBM.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
