| CAT No.BT-CKI-271 |
| Creatine Kinase Muscle Type-1 Human Recombinant, CKMT1 Human, 50ug |
Biological Activity
The biological activity measured by the enzymatic activity of Creatine phosphokinase procedure No.45-UV, 1IU-1 ?mole creatine phosphate was 537IU/mg at 37 degrees celsius corresponding to a Specific Activity of 1,863ng/ml.
Description
CKMT1 Human Recombinant without C-terminal Lysine on both chains produced in Pichia Pastoris is a glycosylated 47kDa polypeptide chain having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and reacts with polyclonal antibodies to MM Isoenzyme in ELISA. The CKMT1 is purified by proprietary chromatographic techniques.
Formulation
CKMT1 contains 0.01M Tris-HCl, 0.075M NaCl, 10mM beta-mercaptoethanol, 50% glycerol, 0.1% sodium azide, pH 7.2.
Sterile Filtered colourless liquid formulation.
Purity
Greater than 95.0% as determined by
a) Analysis by RP-HPLC
b) Analysis by SDS-PAGE
Source
Pichia Pastoris.
Stability
CKMT1 although stable at 15¡ãC for 7 days, should be stored desiccated below -18¡ãC. Please prevent freeze-thaw cycles.
Synonyms
Creatine kinase M-type, EC 2.7.3.2, Creatine kinase M chain, M-CK, CKM, CKMM, CKMMITI.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
