| CAT No.BT-ENZ-073 |
| DNA Topoisomerase-I 70kDa Recombinant Human, TOP1 70kDa Human, 20ug |
Applications
Western blot with Scleroderma patient sera or monoclonal anti-hexa-His-tag antibody.
Coating concentration
0.5-0.8 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Description
Recombinant TOP1 70kDa protein is an enzyme fragment having a molecular mass of 69,199 Dalton (pH 9.4). TOP1 70kDa protein is fused to a hexa-histidine purification tag.
TOP1 70kDa is supplied in 16mM HEPES buffer pH-8.0, 400mM NaCl, and 20% glycerol.
Immunological function
Binds IgG-type human auto-antibodies.
Immunological tests
Standard ELISA test (checkerboard analysis of positive/negative sera panels, including CDC international reference sera).
Purity
Greater than 80% as determined by SDS-PAGE.
Source
Sf9 insect cells.
Storage Conditions
Store at 4¡ãC if entire vial will be used within 2-4 weeks. Store, frozen at -20¡ãC for longer periods of time.
Avoid multiple freeze-thaw cycles.
Synonyms
DNA topoisomerase 1, EC 5.99.1.2, DNA topoisomerase I, TOP1, Scl-70.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
