| CAT No.BT-CHM-332 |
| Exodus-2 Human Recombinant (CCL21), Exodus 2 Human, 20ug |
Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be, Ser-Asp-Gly-Gly-Ala.
Biological Activity
ED50 range = 100-400 ng/mL corresponding to a Specific Activity of 2,500-10,000IU/mg. determined by the chemotactic response of 5-10 day PHA and IL-2 stimulated PBMCs. Optimal concentration for individual application should be determined by a dose response assay.
Description
Exodus-2 Human Recombinant produced in E.Coli as a single, non-glycosylated, polypeptide chain containing 111 amino acids and having a molecular mass of 12219 Dalton.
The CCL21 is purified by proprietary chromatographic techniques.
The CCL21 was lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Solubility
It is recommended to reconstitute the lyophilized Exodus2 in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Source
Escherichia Coli.
Stability
Lyophilized Exodus-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution CCL21 should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
Small inducible cytokine A21, CCL21, Beta chemokine exodus-2, 6Ckine, Secondary lymphoid-tissue chemokine, SLC, chemokine (C-C motif) ligand 21, ECL, CKb9, TCA4, SCYA21, MGC34555.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
