| CAT No.BT-HBV-271 |
| Hepatitis B Virus Protein X Recombinant, HBV X, 25ug |
Applications
Western blotting, ELISA.
Description
17 kDa protein containing 154 amino acid residues.
MAARVCCQLD PARDVLCLRP VGAESRGRPV SGPFGTLPSP SSSAVPADHG AHLSLRGLPV CAFSSAGPCA LRFTSARRME TTVNAHQVLP KVLHKRTLGL SAMSTTDLEA YFKDCLFKDW EELGEEIRLK VFVLGGCRHK LVCSPAPCNFFTSA.
Formulation
Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH4.
Two-step procedure using size exclusion chromatography before and after refolding.
Purity
Greater than 95% as determined by SDS-PAGE.
Solubility
Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10¦Ìg/ml. In higher concentrations the solubility of this antigen is limited.
Source
Escherichia Coli.
Specificity
The amino acid sequence of the recombinant HBx is 100% homologous to amino acid sequence of the native HBx.
Stability
Store lyophilized protein at -20¡ãC. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4¡ãC for a limited period of time; it does not show any change after two weeks at 4¡ãC.
The lyophilized protein remains stable until the expiry date when stored at -20¡ãC.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
