| CAT No.BT-PRO-363 |
| Human Calpain-1 Catalytic Subunit, CAPN1, 5ug |
Applications
This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.
For adsorption we suggest the following procedure:
A- Dilute 1?l of the antiserum against ?-calpain in 1ml of the usual buffer for immunohistochemistry (final dilution 1:1000).
B- Add 1ug of protein to 1ml of the diluted antibody solution and mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections and incubate for 3 days.
E - Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).
As a result, the immunostaining should be strongly reduced or even completely prevented.
Description
CAPN1 consists of an 80-kDa large subunit and a 30 kDa small subunit.
CAPN1 was purified by sequential chromatography through DEAE-Sepharose, A1.5m Bio-Gel, and Phenyl-Sepharose CL-4B columns.
Formulation
50mM imidazole-HCl, 100mM NaCl, 5mM EGTA, 1mM DTT and 10% sucrose.
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Source
Human Erythrocytes.
Stability
CAPN1 although stable at 10¡ãC for 1 week, should be stored desiccated below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
Calpain-1 catalytic subunit, EC 3.4.22.52, Calpain-1 large subunit, Calcium-activated neutral proteinase 1, Calpain mu-type, muCANP, Micromolar-calpain, Cell proliferation-inducing gene 30 protein, CANP 1, CAPN1, CANPL1, PIG30, CANP, muCL, CANP1.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
