Human indoleamine 2,3-dioxygenase,IDO ELISA Kit, 96T
Purpose
This kit is used for the
quantitative determination the concentrations of Indoleamine 2,3-dioxygenase (IDO)
in human
serum,
plasma,
cell culture supernatants,
and other biological fluids.
Principle of the assay
The kit assays
IDO
in the human samples by the method of colorimetric ELISA. After loading of
samples, the
IDO in the
standards or samples interacts with TMB-substrate solution. After incubation,
the reaction is terminated by addition of Stop solution. Determining the
absorbance (OD Value) at wave length 450 nm by the microwell reader, and the
concentration of
IDO
can be
calculated according to standard curve.
Kit Performance
1. Sensitivity: less than 40 pg/ml.
2. Specificity: simultaneous detection of recombinant or natural
IDO
without cross-reaction with other cytokines.
3.
Repeatability: both
inter-assay and intra-assay were less than 10 %.
Storage and validity
Storage:
2-8 ºC. Shelf time: Six months.
*Lead time of this kit is 1-2 weeks. Shipped by
FedEx air.
*Storage: 2 - 8 C. Shelf Time: 6 months.
*Kit Experimental Time:< 2 hs
>>Trial pricing is available for first time use for certain customers,
contact us to see if you are
qualified.
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Important note:
Products are intended for laboratory or
manufacture use and are not to be used for diagnosis or any other purpose, which
include but are not limited to, unauthorized commercial uses, ex vivo or in
vivo therapeutic uses. Not for Human or Animal Consumption.
