Leptin Mouse Recombinant, Leptin Mouse, 1mg

Amino acid sequence
MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC.

Biological Activity
Biological activity of Mouse Leptin is performed in two different mouse obesity models, ob/ob and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5ug Leptin/gram body weight for a period of 14 days. Significant effects on body weight , food consumption, and plasma glucose levels were observed to saline-treated controls.

Description
Leptin Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16.2 kDa.
The Leptin is purified by proprietary chromatographic techniques.

Formulation
The mouse Leptin was lyophilized from a concentrated (1mg/ml) solution containing 0.1% TFA.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Protein content
Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.201 as the extinction
coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Leptin as a Reference Standard.

Purity
Greater than 95.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Leptin in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution Leptin should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
OB Protein, Obesity Protein, OBS, Obesity factor.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.