| CAT No.BT-PRO-539 |
| Lipopolysaccaride Mouse Recombinant, LBP Mouse, 5ug |
Applications
? ELISA
? Western Blotting.
Characterization
On SDS-PAGE commassie blue stained gel, the 95% purified recombinant protein shows a band at 58 kDa.
Description
LBP is produced from mouse LBP transfected CHO-cells in serum free medium. For transfection we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before tranfection the complete mouse LBP cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. RmLBP was produced by his-tag by means of metal affinity purification with Talon and controlled by SDS page.
Attention: His-tag at the c-terminal end of the LBP has no protease site and is not to split off. up to 2ug/ml LBP mediates binding of FITC-LPS (0.5ug/ml) to CD14+CHO transfectants (2 x 1,000,000/ml).
Recombinant Mouse LBP was lyophilized from a protein solution (1 mg/ml) containing phosphate-buffered saline, pH 7.2.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility
It is recommended to reconstitute the lyophilized LBP in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Source
Chinese Hamster Ovarian Cells (CHO).
Stability
Lyophilized LBP Mouse Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution LBP should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
Please prevent freeze-thaw cycles.
Synonyms
Lipopolysaccharide-binding protein, LBP, Ly88.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
