| CAT No.BT-PRO-320 |
| Listeriolysin-O Recombinant, LLO, 50ug |
Activity
LLO has a hemolytic activity 1.25x106 HU/mg.
2mM DTT is used to reactivate the toxin.
Applications
a. Cytosolic delivery of molecules, peptides, oligonucleodides and plasmid DNA (4).
b. Production of specific monoclonal antibodies (5).
c. Detection of anti-listeriolysin O antibodies (6).
Description
Molecular weight of Listeriolysin O is 58kDa. This haemolysin protein is provided in its mature form that is constituted by 503 amino acids, from 26 to 529 of the registered sequence (accession number: AAF64524).
The protein contains 50mM NaH2PO4, 1mM EDTA, 2.7mM KCl, 1mM DTT, 5% glycerol and 0.5M NaCl.
Physical Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Source
Escherichia Coli.
Stability
LLO although stable at room temperature for 1 week, should be stored desiccated below -18¡ãC.
Please prevent freeze-thaw cycles.
Synonyms
Listeriolysin-O, LLO, hlyA.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
