| CAT No.BT-ENZ-269 |
| Lysostaphin Recombinant, Lysostaphin, 5mg |
Biological Activity
Determined by the decrease in turbidity of a suspension of heat-killed Staphylococcus aureus at pH8.0, 30¡ãC.
Description
Lysostaphin, an endopeptidase specific for the cell wall peptidoglycan of staphylococci, is an extremely potent anti-staphylococcal agent. Lysostaphin is used as a research and diagnostic tool. Because it lyses staphylococci efficiently, it is widely used when preparing staphylococcal DNA or other cellular components for genetic and biochemical studies and for the preparation of protoplasts for transformation. Preparation and analysis of bacterial DNA has become a powerful tool used by clinical and other microbiologists in epidemiological studies aimed at tracing sources of infection or bacterial contamination.
Formulation
The protein was lyophilized without any additives.
Sterile Filtered lyophilized powder.
Protein content
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 2.02 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis RP-HPLC, using a calibrated solution of Lysostaphin as a Reference Standard.
Purity
96.9% as determined by RP-HPLC.
Solubility
It is recommended to reconstitute the lyophilized Lysostaphin in 20mM sodium acetate, pH 4.5, which can then be further diluted to other aqueous solutions.
Source
Escherichia Coli.
Specific Activity
Determined to be 4,243 units/mg.
Stability
Lysostaphin although stable at 4¡ãC for 6 months, should be stored desiccated below -18¡ãC.
Please prevent freeze-thaw cycles.
Synonyms
Lysostaphin, EC 3.4.24.75, Glycyl-glycine endopeptidase.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
