Important note: Products are intended for laboratory use and are not to be used for any other purpose, which include but are not limited to, unauthorized commercial uses, ex vivo or in vivo therapeutic uses. Not for Human or Animal Consumption.
Cat NO. CL047
Mouse NIH 3T3+Vanadate+PDGF
Lysate
FOR
RESEARCH USE ONLY
Volume: 500 ?g
l Storage
?
Store undiluted at -20 ?C for long term storage
?
Store undiluted at 4 ?C for short time storage (up to one month)
?
Stable
at RT up to one week
?
Do
not dilute or boil
l Description
Cell
or tissue lysates are a useful control for the western blotting application. As
a source that can be rich in endogenously expressed proteins, this lysate is
convenient to be used and provided in a denatured format and allowing for
immediately loading onto an SDC-PAGE gel.
This
lysate was routinely tested by western blot analysis.
l Preparation
NIH
3T3 cells were treated by serum starved overnight and then incubated at RT for
10 min in a final concentration of 1 mM sodium vanadate. PDGF was then added at
a final concentration of 5 ng/ml and cells were incubated at 37 ?C for 30 min.
Western blots of NIH 3T3 cell lysates treated with vanadate and PDGF have been
reported to show a shift in SHC mobility in addition to phosphorylation of
PKBα/Akt and Stat3 as compared to untreated control cells.
l Application
Western Blot
l Product Notice
Due to variable
applications, each investigator should titrate the reagent to obtain optimal
results.
