| CAT No.BT-CYT-341 |
| Platelet-Derived Growth Factor AA Human Recombinant, PDGF AA Human, 10ug |
Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ile-Glu-Glu-Ala.
Biological Activity
The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake) is < 1 ng/ml, corresponding to a Specific Activity of 1 MIU/mg.
Description
Platelet-Derived Growth Factor AA Human Recombinant is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 125 amino acids and having a total molecular mass of 28511 Dalton.
PDGF-AA is purified by proprietary chromatographic techniques.
The protein was lyophilized with no additives.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility
It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-AA in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Source
Escherichia Coli.
Stability
Lyophilized Platelet-Derived Growth Factor AA although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution PDGF-AA should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AA, PDGF-1.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
