| CAT No.BT-ENZ-262 |
| Protein Disulfide Isomerase Human Recombinant, PDI Human, 500ug |
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Arg-Ala.
Description
PDI Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 503 amino acids and having a molecular mass of 62.4 kDa. The PDI is fused to a 12 amino acid His tag (515 a.a. total) at N-terminal and purified by proprietary chromatographic techniques.
Formulation
The PDI protein (1mg/ml)solution was lyophilized from PBS pH-7.
0.5 ?mol active RNase A min-1 ?mol PDI-1. According to the re-activation of reduced and denatured RNase A (Lyles, M. M. and Gilbert, H. F. (1991) Biochemistry 30, 613-619).
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by
a) Analysis by RP-HPLC.
b) Analysis by SDS-PAGE.
Reductase Activity
0.001 650nm/ min-2. By measuring the turbidity increase at 650 nm due to insulin reduction (Holmgren, A. (1979) J. Biol. Chem. 254, 9627¨C9632). The activity is expressed as the ratio of the slope of a linear part of the turbidity curve to the lag time (Mart??nez-Galisteo, E., Padilla, C. A., Garcia-Alfonso, C., Lo?pez-Barea, J., and Barcena, J. A. (1993) Biochimie (Paris) 75, 803¨C809).
Solubility
It is recommended to reconstitute the lyophilized PDI in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Source
Escherichia Coli.
Stability
Lyophilized Protein Disulfide Isomerase although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution Human PDI should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Synonyms
Protein Disulfide Isomerase, PDI, EC 5.3.4.1, Prolyl 4-hydroxylase subunit beta, Cellular thyroid hormone-binding protein, p55, P4HB, ERBA2L, PDIA1, PO4DB, DSI, GIT, PHDB, PO4HB, PROHB, P4Hbeta.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
