| CAT No.BT-PKA-207 |
| Protein Kinase Akt1/PKB alpha, Inactive enzyme Human Recombinant, PKAkt1/PKBa Human, 20ug |
Description
PKAkt1 is a glycosilated polypeptide having a molecular mass of 59.1 kDa, fused with a polyhistidine tag at N-terminus (to facilitate removal of Akt1 kinase from the reaction mixture).
Inactive enzyme, suitable for negative control experiments or for phosphorylation as a substrate.
Recombinant Protein Kinase B is purified by proprietary chromatographic techniques.
Unit definition
1 Unit is defined as 1 picomole phosphate transferred to the synthetic peptide (RPRAATF) per min at 30¡ãC.
Activity
No protease activity (Twinning test).
The Specific activity is 235 U/mg.
PKAkt1 1.9mg/ml, in 50mM Tris-HCl, 100mM NaCl, 1mM DTT, 25mM beta glycerophosphate, 50% glycerol, pH 8.5.
Physical Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Source
Sf9 insect cells.
Storage Conditions
Store at 4¡ãC if entire vial will be used within 2-4 weeks.
Store, frozen at -20¡ãC for longer periods of time.
Avoid multiple freeze-thaw cycles.
Synonyms
RAC-alpha serine/threonine-protein kinase, EC 2.7.11.1, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, AKT1, AKT, RAC, PRKBA, MGC99656, RAC-ALPHA.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
