RANK Ligand Soluble Human Recombinant, sRANKL Human, 10ug

Purity
Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Lys-Ala-Met.

Biological Activity
The activity is determined by its ability to induce osteoclast formation in RAW264.7 cells using a conc. of 5.0-10.0 ng/ml corresponding to a specific activity of 100,000-200,000 Units/mg and by dose dependant stimulation of IL-8 production in human PBMC.

Description
sRANKL Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 20006 Dalton.
CD254 is purified by proprietary chromatographic techniques.

Formulation
The protein was lyophilized from a concentrated (1mg/ml) solution containing 10mM Tris, pH-7.5.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Solubility
It is recommended to reconstitute the lyophilized sRANKL in sterile 18M¦¸-cm H2O at a concentration of 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized TNFSF11 although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution sRANKL should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
Soluble Receptor Activator of NFkB Ligand, TNFSF11, TRANCE, TNF-related activation-induced cytokine, OPGL, ODF, Osteoclast differentiation factor, Tumor necrosis factor ligand superfamily member 11, Receptor activator of nuclear factor kappa B ligand, RANKL, Osteoprotegerin ligand, CD254 antigen, sRANKL, sOdf, hRANKL2.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.