| CAT No.BT-ANT-157 |
| SARS-Spike protein, Polyclonal Antibody, SARS-Spike Antibody, 200ug |
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Western blot analysis of Spike in (A) transfected and (B) untransfected mouse melanoma cell lysate at a concentration of 0.05 ug/ml.
Antigen
The antibody was developed by immunizing rabbits with synthetic peptides corresponding to amino acids 1124-1140 of the putative SARS Spike glycoprotein (Genbank accession no. NP_828851).
Applications
This polyclonal antibody can be used for detection of SARS Spike protein in Western blot. Full-length Spike protein transfected into UM92 cells was used as a positive control and an approximate 139KDa band is detected.
It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.
Contents
100ugs in 200?l PBS containing 0.2% gelatin and 0.05% sodium azide.
Isotype
Rabbit IgG.
Type
Polyclonal Rabbit Antibody.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
