| CAT No.BT-ENZ-290 |
| Secreted Phospholipase A2-IIA Human Recombinant, PLA2G2A Human, 10ug |
Purity
Greater than 95% as determined by SDS-PAGE.
Applications
Western blotting.
Description
Secreted Phospholipase A2-IIA Human Recombinant is manufactured with N-terminal fusion of HisTag. PLA2G2A His-Tagged Fusion Protein is 15.8 kDa containing 124 amino acid residues of the human secreted phospholipase A2-IIA and 16 additional amino acid residues ¨C HisTag (underlined).
MRGSHHHHHH GMASHMNLVN FHRMIKLTTG KEAALSYGFY GCHCGVGGRG SPKDATDRCC VTHDCCYKRL EKRGCGTKFL SYKFSNSGSR ITCAKQDSCR SQLCECDKAA ATCFARNKTT YNKKYQYYSN KHCRGSTPRC.
Lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.
Physical Appearance
Sterile Filtered lyophilized (freeze-dried) powder.
Purification Method
Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.
Solubility
Add 0.2 ml of 0.1M Acetate buffer pH-4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 ¦Ìg/ml. In higher concentrations the solubility of this antigen is limited.
Source
Escherichia Coli.
Specificity
The amino acid sequence of the PLA2G2A is 100% homologous to the amino acid sequence of the human Secreted Phospholipase A2-IIA.
Stability
Store lyophilized protein at -20¡ãC. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4¡ãC for a limited period of time; it does not show any change after two weeks at 4¡ãC.
The lyophilized protein remains stable until the expiry date when stored at -20¡ãC.
Synonyms
MOM1, PLA2, PLA2B, PLA2L, PLA2S, PLAS1, sPLA2, Phospholipase A2 membrane associated, EC 3.1.1.4, Phosphatidylcholine 2-acylhydrolase, Group IIA phospholipase A2, GIIC sPLA2, Non-pancreatic secretory phospholipase A2, NPS-PLA2, sPLA2-IIA, PLA2G2A.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
