| CAT No.BT-ENZ-330 |
| Secreted Phospholipase A2-XII Human Recombinant, PLA2G12 Human, 10ug |
Applications
Western blotting.
Description
Secreted Phospholipase A2-XII Human Recombiannt was produced with N-terminal His-Tag. PLA2G12 His-Tagged Fusion Protein is 20.6 kDa containing 167 amino acid residues of the human secreted phospholipase A2-XII and 16 additional amino acid residues ¨C His-Tag (underlined).
MRGSHHHHHHGMASHMQEQAQTTDWRATLKTIRNGVHKIDTYLNAALDLL
GGEDGLCQYKCSDGSKPFPRYGYKPSPPNGCGSPLFGVHLNIGIPSLTKCCNQ
HDRCYETCGKSKNDCDEEFQYCLSKICRDVQKTLGLTQHVQACETTVELLFD
SVIHLGCKPYLDSQRAACRCHYEEKTDL.
Formulation
Sterile filtered and lyophilized from 0.5 mg/ml in 0.01M Tris buffer pH 8.6.
Sterile Filtered lyophilized (freeze-dried) powder.
Purification Method
Ni-NTA affinity chromatography.
Purity
Greater than 95% as determined by SDS PAGE.
Solubility
Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely.
Source
Escherichia Coli.
Specificity
The amino acid sequence of the recombinant human Secreted Phospholipase A2-XII is 100% homologous to the amino acid sequence of the human Secreted Phospholipase A2-XII without signal sequence.
Stability
Store lyophilized protein at -20¡ãC. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4¡ãC for a limited period of time; it does not show any change after two weeks at 4¡ãC.
The lyophilized protein remains stable until the expiry date when stored at -20¡ãC.
Synonyms
Group XIIA secretory phospholipase A2, EC 3.1.1.4, Phosphatidylcholine 2-acylhydrolase GXII, GXII sPLA2, PLA2G12, sPLA2-XII, PLA2G12A.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
