Vascular Endothelial Growth Factor (121 a.a.) Human Recombinant, Sf9, VEGF (121 a.a.) Human, Sf9, 10ug

Biological Activity
Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 2-5ng/ml corresponding to a Specific Activity of 200,000-500,000IU/mg.

Description
Vascular Endothelial Growth Factor-121 Human Recombinant produced in insect cells as an 18kDa homodimer, is a glycosylated, polypeptide chain containing 121 amino acids and having a molecular mass of approximately 36kDa.
VEGF121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates.
The VEGF-121 is purified by proprietary chromatographic techniques.

Formulation
The protein was lyophilized from a concentrated (1mg/ml) solution with no additives.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 90.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility
The lyophilized Vascular Endothelial Growth Factor -121 is soluble in water and most aqueous buffers. The lyophilized VEGF-121 should be reconstituted in PBS or medium containing at least 0.1% HSA or BSA to a concentration not lower than 50ug/ml.

Source
Sf9, Insect Cells.

Stability
Lyophilized Vascular Endothelial Growth Factor 121 although stable at room temperature for 3 weeks, should be stored desiccated below
-18¡ãC. Upon reconstitution VEGF-121 should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.