| CAT No.BT-PRO-309 |
| Vimentin Human Recombinant, Vimentin Human, 10ug |
Description
Vimentin Human Recombinant is produced in E.Coli, having a molecular weight of 53,685 dalton (calaulated from sequence), 57,000 dalton (determinated by SDS gelelctrophoresis).
Formulation
The protein (1mg/ml) was lyophilized with 30mM Tris/HCL, pH 8, 9.5M urea, 2mM EDTA, 2mM DTT 10mM methylammonium chloride.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95.0% as determined by SDS-gelelctrophoresis.
Reconstitution to filaments
After vimentin is dissolved in 9.5M urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
- Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841
- Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255.
Solubility
It is recommended to reconstitute the Vimentin in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Source
Escherichia Coli.
Stability
Lyophilized Vimentin although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution Vimentin should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
Vimentin, Vim, FLJ36605.
Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
