Angiopoietin-like Protein 4 Human Recombinant, ANGPTL4 Human, 10ug

Applications
Western blotting, ELISA.

Description
The ANGPTL4 Human Recombinant is manufactured with N-terminal fusion of His Tag.
The Angiopoietin-like Protein 4 His -Tagged Fusion Protein is 25 kDa protein containing 204 amino acid residues of the Angiopoietin-like Protein 4 and 16 additional amino acid residues - His Tag (underlined).
MRGSHHHHHH GMASHMGPVQ SKSPRFASWD EMNVLAHGLL QLGQGLREHA ERTRSQLSAL ERRLSACGSA CQGTEGSTDL PLAPESRVDP EVLHSLQTQL KAQNSRIQQL FHKVAQQQRH LEKQHLRIQH LQSQFGLLDH KHLDHEVAKP ARRKRLPEMA QPVDPAHNVS RLHRLPRDCQ ELFQVGERQS GLFEIQPQGS PPFLVNCKMT SDGGWTVIQR.

Formulation
Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.

Purification Method
One-step procedure using affinity Ni-NTA chromatography.

Purity
Greater than 95% as determined by SDS-PAGE.

Solubility
Add 0.2 ml of 0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10¦Ìg/ml. In higher concentrations the solubility of this antigen is limited.

Source
Escherichia Coli.

Specificity
The amino acid sequence of the ANGPTL4 Human recombinant is 100% homologous to the 26-229 amino acid sequence of the Angiopoietin-like Protein-4 precursor without signal sequence.

Stability
Store lyophilized Angiopoietin-like Protein 4 Human recombinant at
-20¡ãC. Aliquot the ANGPTL4 after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted ANGPTL4 can be stored at
4¡ãC for a limited period of time; it does not show any change after two weeks at 4¡ãC. The lyophilized ANGPTL4 remains stable until the expiry date when stored at -20¡ãC.

Synonyms
ANGPTL4, NL2, ARP4, FIAF, PGAR, HFARP, pp1158, ANGPTL2, Fasting- Induced Adipose Factor, Hepatic Fibrinogen/Angiopoietin-Related Protein, PPARG Angiopoietin-Related Protein.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.