Bone Morphogenetic Protein-4 Human Recombinant, BMP 4 Human, 10ug

Amino acid sequence
SPKHHSQRAR KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR.

Applications
1. Molecular standard (Western, ELISA) in studying secreted BMP-4.
2. Preparing antibodies for BMP-4 monomer.
3. Molecule standard in detecting secreted BMP-4 in reduced SDS-PAGE.

Description
Bone Morphogenetic Protein-4 Human Recombinant produced in E.Coli is a monomeric, non-glycosylated, Polypeptide chain containing 116 amino acids and having a molecular mass of 13009 Dalton.
The BMP-4 is purified by proprietary chromatographic techniques.

Formulation

BMP-4 was lyophilized from a 0.2?m filtered concentrated (1mg/ml) solution in 30% Acetonitrile and 0.1% TFA.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-4 in sterile 100mM AcOH (acetic Acid) not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized Bone Morphogenetic Protein-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution BMP4 should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
BMP4, ZYME, BMP2B, BMP2B1.

Usage
ProsSec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.