Complement Component C5a Mouse Recombinant, C5a Mouse, 10ug

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Leu-His-Leu-Leu.

Biological Activity

The ED50 of Recombinant Mouse C5a as determined by its ability to induce N-acetyl-b-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells was 5-20ng/ml.

Description

C5a Mouse Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 77 amino acids and having a molecular mass of 9kDa.
The Mouse C5a is purified by proprietary chromatographic techniques.

Mouse C5a was lyophilized from a concentrated (1mg/ml) solution in 20mM PB, pH 7.5 and 350mM NaCl.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized Mouse C5a in sterile 18M?-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized Mouse C5a although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution Mouse C5a should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
Please prevent freeze-thaw cycles.

Synonyms

Complement C5, Hemolytic complement, C5, Hc, He, C5a.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.