Cystatin-A Human Recombinant, CSTA Human, 10ug

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ile-Pro-Gly-Gly.

Biological Activity

The IC50 value is < 3.4 nM, as measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate.

Description
Cystatin A Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 98 amino acids and having a molecular mass of 11kDa.
The Cystatin A is purified by proprietary chromatographic techniques.

Cystatin A was lyophilized from a concentrated (1mg/ml) solution in 50mM Tris-HCl, pH 7.5 and 100mM NaCl.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized Cystatin A in sterile 18M?-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized Cystatin A although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution CSTA should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
Please prevent freeze-thaw cycles.

Synonyms
Cystatin-A, Cystatin-AS, Stefin-A, CSTA, STF1, STFA.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.