Dihydrofolate Reductase Human Recombinant, DHFR Human, 50ug

CAT No.BT-ENZ-443

Dihydrofolate Reductase Human Recombinant, DHFR Human, 50ug

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Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MVGSLNCIVA VSQNMGIGKN GDLPWPPLRN EFRYFQRMTT TSSVEGKQNL VIMGKKTWFS IPEKNRPLKG RINLVLSREL KEPPQGAHFL SRSLDDALKL TEQPELANKV DMVWIVGGSS VYKEAMNHPG HLKLFVTRIM QDFESDTFFP EIDLEKYKLL PEYPGVLSDV QEEKGIKYKF EVYEKND.

Biological activity
Specific activity is 1.5 - 2.5 units/ml and was obtained by measuring the oxidation of NADPH in absorbance at 340 nm during reaction. One unit will convert 1.0 umole of 7,8 dihydrofolate and beta-NADPH to 5,6,7,8-tetrahydrofolate and beta-NADP per minute at pH 6.5 at 25C.

Description
DHFR Human Recombinant fused with 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 207 amino acids (1-187 a.a.) and having a molecular mass of 23.6kDa.
The DHFR is purified by proprietary chromatographic techniques.

The DHFR solution contains 20mM Tris-HCl buffer (pH8.0), 0.1M Nacl 2mM DTT, and 30% glycerol.

Physical Appearance
Sterile Filtered colorless solution.

Purity
Greater than 95.0% as determined by SDS-PAGE.

Source
Escherichia Coli.

Stability
DHFR although stable 4¡ãC for 4 weeks, should be stored desiccated below -18¡ãC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
Dihydrofolate reductase, DHFR, DHFRP1.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.