Dipeptidyl-Peptidase 4 Human Recombinant, DPP4 Human, 10ug

CAT No.BT-ENZ-375

Dipeptidyl-Peptidase 4 Human Recombinant, DPP4 Human, 10ug

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Amino Acid Sequence
ADP-SRKTYTLTDYLKNTYRLKLYSLRWISDHEYLYKQENN
ILVFNAEYGNSSVFLENSTFDEFGHSINDYSISPDGQ
FILLEYNYVKQWRHSYTASYDIYDLNKRQLITEERIP
NNTQWVTWSPVGHKLAYVWNNDIYVKIEPNLPSYRIT
WTGKEDIIYNGITDWVYEEEVFSAYSALWWSPNGTFL
AYAQFNDTEVPLIEYSFYSDESLQYPKTVRVPYPKAG
AVNPTVKFFVVNTDSLSSVTNATSIQITAPASMLIGD
HYLCDVTWATQERISLQWLRRIQNYSVMDICDYDESS
GRWNCLVARQHIEMSTTGWVGRFRPSEPHFTLDGNSF
YKIISNEEGYRHICYFQIDKKDCTFITKGTWEVIGIE
ALTSDYLYYISNEYKGMPGGRNLYKIQLSDYTKVTCL
SCELNPERCQYYSVSFSKEAKYYQLRCSGPGLPLYTL
HSSVNDKGLRVLEDNSALDKMLQNVQMPSKKLDFIIL
NETKFWYQMILPPHFDKSKKYPLLLDVYAGPCSQKAD
TVFRLNWATYLASTENIIVASFDGRGSGYQGDKIMHA
INRRLGTFEVEDQIEAARQFSKMGFVDNKRIAIWGWS
YGGYVTSMVLGSGSGVFKCGIAVAPVSRWEYYDSVYT
ERYMGLPTPEDNLDHYRNSTVMSRAENFKQVEYLLIH
GTADDNVHFQQSAQISKALVDVGVDFQAMWYTDEDHG
IASSTAHQHIYTHMSHFIKQCFSLP-SGRLVPRGSHHHHHH.

Assay Methods
- Reaction buffer: 20mM Tris pH8.0, 0.1M NaCl, 1mM EDTA
- Total reaction volume: 100 ul
- Reaction temperature: 37¡ãC. 1. Add the reaction buffer to each well.
2. Add the 10 ul of 10mM substrate (Gly-Pro p-nitroanilide) to each well.
3. Add the enzyme (DPP-4) diluent to each well.
4. Incubate the 96 well plate at 37¡ãC.
5. Read the optical density at 405 nm. Optical density was measured at 405 nm after incubating enzyme solution with 1mM of p-nitroanilid as a substrate. 0ng DPP4= 0 OD at 405nm.
20ng DPP4= 0.075 OD at 405nm.
40ng DPP4= 0.125 OD at 405nm.
60ng DPP4= 0.2 OD at 405nm.
80ng DPP4= 0.275 OD at 405nm.
100ng DPP4= 0.35 OD at 405nm.

Biological Activity
>20 Units/mg.

DPPIV Human Recombinant produced in High-5 cells is a single, glycosylated polypeptide chain containing 746 amino acids (39-766) and having a molecular mass of 86.4 kDa.
DPPIV is fused to His Tag at C-terminus and purified using conventional chromatography techniques.

Formulation
DPP4 is formulated in 20mM Tris-HCl buffer pH-8, 100mM NaCl, 1mM EDTA and 10% glycerol.

Physical Appearance
Sterile Filtered colorless solution.

Purity
Greater than 95.0% as determined by Analysis by SDS-PAGE.
On SDS-PAGE under denatured condition, apparent molecular weight of glycosylated DPP4 will migrate at approximately 90kDa.

Source
High-5 cells.

Stability
DPP4 although stable 4¡ãC for 4 weeks, should be stored desiccated below -18¡ãC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
CD26, ADABP, ADCP2, DPPIV, TP103, DPP4, Dipeptidyl peptidase 4, Dipeptidyl peptidase IV, DPP IV, T-cell activation antigen CD26, Adenosine deaminase complexing protein 2, CD26 antigen.

Unit Definition
One unit will hydrolyze 1 mmole of p-nitroaniline per minute at pH8.0 at 37¡ãC using 1mM of Gly-Pro p-nitroanilde as a substrate.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.