Fibroblast Growth Factor Acidic Bovine, aFGF Bovine, 5ug

CAT No.BT-CYT-613

Fibroblast Growth Factor Acidic Bovine, aFGF Bovine, 5ug

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Biological Activity
Stimulates growth of bovine capillary endothelial cells by 3-5 fold over 5% calf serum at 10-25ng/ml FGF and 0ug/ml heparin.

Description
Fibroblast Growth Factor-acidic Bovine (FGF-1) purified from Bovine Brain contains a 17 kDa and a 20 kDa polypeptide chain. The 17 kDa peptide is derived from the 20K peptide by restricted proteolysis. (See Jaye et al?). The FGF acidic is purified by proprietary chromatographic techniques.

Formulation
Each 5ug aFGF were lyophilized from 0.5ml solution containing 1mM sodium phosphate, pH 7 after filtration over a low binding membrane.

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 90%.

Solubility
It is recommended to reconstitute the lyophilized aFGF in sterile 50mM Na2HPO4 pH-7, and 0.5% albumin. The Recommended concentration in cell culture: 1-20ng/ml.

Source
Bovine Brain.

Stability
Lyophilized aFGF although stable at room temperature for 2 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution aFGF should be stored at 4¡ãC between 2-3 weeks and for future use below
-18¡ãC.
Please prevent freeze-thaw cycles.

Synonyms
HBGF-1, ECGF-beta, FIBP, FGFIBP, FIBP-1, ECGF, ECGFA, GLIO703, FGF1, FGF-a.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.