Fibroblast Growth Factor Basic 147 a.a. Human Recombinant, FGF 2 Human (147 a.a.), 50ug

CAT No.BT-CYT-557

Fibroblast Growth Factor Basic 147 a.a. Human Recombinant, FGF 2 Human (147 a.a.), 50ug

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Amino acid sequence
MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS

Biological Activity
The ED50, Calculated by the dose-dependant proliferation of mouse BALB/c 3T3 cells is 0.12 ng/ml, corresponding to a specific activity of 8,333,333 Units/mg.

Description
Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16539 Dalton.
The FGF2 is purified by proprietary chromatographic techniques.

The bFGF was lyophilized from a concentrated sterile solution containing 10mM sodium phosphate buffer pH=8.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized FGF-B in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized basic-FGF although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution FGFb should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.