Fibroblast Growth Factor-basic Rat Recombinant, FGF 2 Rat, 50ug

CAT No.BT-CYT-608

Fibroblast Growth Factor-basic Rat Recombinant, FGF 2 Rat, 50ug

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Amino acid sequence
PALPEDGGGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHV KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTEE CFFFERLESN NYNTYRSRKY SSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS.

Biological Activity
The ED50 range as determined by the dose-dependant proliferation of BALB/3T3 cells was found to be less than 0.2 ng/ml, corresponding to a specific activity of 5,000,000 IU/mg.

Description
bFGF Rat Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 145 amino acids and having a molecular mass of 16.3 kDa.
The bFGF2 is purified by proprietary chromatographic techniques.

FGF-b was lyophilized from 1 mg/ml solution after extensive dialysis against 20mM phosphate buffer, pH 7.4 and 130mM NaCl.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 97.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Rat bFGF in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Escherichia Coli.

Stability
Lyophilized Rat bFGF although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution Rat FGF-2 should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
HBGH-2, HBGF-2, Prostatropin, FGF-2, FGB-b, Fibroblast Growth Factor-basic, Basic fibroblast growth factor, bFGF, Heparin-binding growth factor 2.

Usage
TSZGENE's products are furnished form LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.