Flt3-Ligand Human Recombinant, HEK derived, Flt3 Ligand Human, HEK, 10ug

CAT No.BT-CYT-706

Flt3-Ligand Human Recombinant, HEK derived, Flt3 Ligand Human, HEK, 10ug

---

Amino acid sequence
TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTAPQP.

Biological Activity
The ED50 as determined by the dose dependent proliferation of human OCI-AML5 cells is 0.5 to 1.0 ng/ml (Specific Activity: 2.0 x 1,000,000 ¨C 1.0 x 1,000,000 units/mg).

Description
Flt3-Ligand Human Recombinant produced in HEK293 cells is a glycosylated, polypeptide chain, migrates as a diffuse band on SDS-PAGE due to heterogeneous glycosylation at 30kDa. Flt3-Ligand is purified by proprietary chromatographic techniques.

The protein was lyophilized with no additives.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Flt3-Ligand in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
HEK293 (Human Embryonic Kidney cell line).

Stability
Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution Flt3-Ligand should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Synonyms
Fms-related tyrosine kinase 3 ligand, FLK2, STK1, CD135, Stem Cell Tyrosine Kinase 1, FLT3LG, Flt3.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.