Flt3 Ligand Human Recombinant, Sf9, Flt3 Ligand Human, Sf9, 10ug

CAT No.BT-CYT-409

Flt3 Ligand Human Recombinant, Sf9, Flt3 Ligand Human, Sf9, 10ug

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Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Gln-Asp-Cys-Ser.

Biological Activity
The ED50, calculated by the dose-dependant stimulation of the proliferation of human MUTZ-2cells is < 3.0 ng/ml corresponding to a Specific Activity of 0.34MIU/mg.

Description
Flt3-Ligand Human Recombinant produced in Sf9 insect cells is a glycosylated, polypeptide chain containing 155 amino acids and having migrating on SDS-PAGE at 19kDa.
Flt3-Ligand is purified by proprietary chromatographic techniques.

The protein was lyophilized with no additives.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Protein content
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 1.42 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a standard of Flt3-Ligand as a Reference Standard.

Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility
It is recommended to reconstitute the lyophilized Flt3-Ligand in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
Baculovirus Sf9 cells.

Stability
Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution Flt3-L should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms
Fms-related tyrosine kinase 3 ligand, FLK2, STK1, CD135, Stem Cell Tyrosine Kinase 1, FLT3LG, Flt3.

Usage

Amino acid sequence
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.