Frizzled-1 Human Recombinant, FZD1 Human, 10ug

CAT No.BT-PRO-816

Frizzled-1 Human Recombinant, FZD1 Human, 10ug

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Amino acid sequence

DHGYCQPISI PLCTDIAYNQ TIMPNLLGHT NQEDAGLEVH QFYPLVKVQC SAELKFFLCS MYAPVCTVLE QALPPCRSLC ERARQGCEAL MNKFGFQWPD TLKCEKFPVH GAGELCVENL YFQGGSGTKL DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHGD PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGK.

Biological Activity

The K_D (equilibrium binding constant) of FZD1 Human was measured at less than 10nM with human Wnt3a as the ligand. The measurements were performed by the ForteBio Octet optical biosensor instrument.

Description

Frizzled-1 Human Recombinant produced in HEK293 cells, corresponding to the extracellular region of mature Human Frizzled-1 (amino acids 112-228) and fused to the Fc region of Human IgG1, having an MW of 46kDa.
Frizzled-1 is purified by proprietary chromatographic techniques.

The protein was lyophilized without any additives.

Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized FZD1 in sterile 1xPBS not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source
HEK293 (Human Embryonic Kidney cell line).

Stability

Lyophilized FZD1 although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution FZD1 should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Frizzled-1, Fz-1, hFz1, FzE1, FZD1, FLJ95923, DKFZp564G072.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.