Gliadin Wheat Recombinant, Gliadin Wheat, 20ug

CAT No.BT-pro-114

Gliadin Wheat Recombinant, Gliadin Wheat, 20ug

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Applications
Western blot with monoclonal anti-hexa-His-tag antibody.

Coating concentration
0.15-0.5 ug/ml (depending on the type of ELISA plate and coating buffer).

Description
Gliadin is a cDNA coding for an epitope-carrying fragment of a wheat gamma-gliadin isoform, having a molecular mass of 18,616 Dalton (high proline content and the low pI are likely causes for the observed discrepancy between calculated molecular weight and the observed electrophoretic mobility of approx. 50 kDa on standard SDS-PAGE), pH 4.6. By sequence design the epitopes correspond to the deamidated neo-epitopes, which in the natural antigen are formed by transglutaminase-mediated glutamine side chain deamidation. Gliadin protein is fused to a hexa-histidine purification tag.

Gliadin is supplied in 16mM HEPES buffer pH-8.0, 400mM NaCl, and 20% glycerol.

Immunological function
Binds IgG- and IgA-type human autoantibodies associated with celiac disease.

Immunological tests
Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot.

Purity
Greater than 80% as determined by SDS-PAGE.

Source
Escherichia Coli.

Storage Conditions
Store at 4¡ãC if entire vial will be used within 2-4 weeks.
Store, frozen at -20¡ãC for longer periods of time.
Avoid multiple freeze-thaw cycles.

Usage
TSZGENE's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.